Improving precision of proximity ligation assay by amplified single molecule detection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23874999.
- Also identified by DOI 10.1371/journal.pone.0069813 and PMC identifier 3713053.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.
Medical subject headings
- Biological Assay