Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains.
basic_science · Level V
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- Record sourced from PubMed, PMID 23878250.
- Also identified by DOI 10.1073/pnas.1222041110 and PMC identifier 3740907.
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Abstract
Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.
Medical subject headings
- Acridones
- Fluorescent Dyes
- Mycobacterium
- Sulfatases
- Sulfates
- Sulfuric Acid Esters