Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23891978.
- Also identified by DOI 10.1053/j.gastro.2013.07.026 and PMC identifier 3805793.
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Abstract
The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection.
Medical subject headings
- Disease Models, Animal
- Hepacivirus
- Hepatitis C
- Hepatocytes
- Induced Pluripotent Stem Cells
- Macaca nemestrina