RNA-guided gene activation by CRISPR-Cas9-based transcription factors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23892895.
- Also identified by DOI 10.1038/nmeth.2600 and PMC identifier 3911785.
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Abstract
Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Protein Engineering
- RNA Editing
- Transcription Factors
- Transcriptional Activation