Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23894349.
- Also identified by DOI 10.1371/journal.pone.0068824 and PMC identifier 3720884.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.
Medical subject headings
- Base Composition
- Genome, Bacterial
- Genomics
- High-Throughput Nucleotide Sequencing
- Lichens
- Streptomyces