The transience of transient overexpression.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23900254.
- Also identified by DOI 10.1038/nmeth.2534.
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Abstract
Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.
Medical subject headings
- Gene Expression Regulation
- Genome
- Transfection