Visualizing SNVs to quantify allele-specific expression in single cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23913259.
- Also identified by DOI 10.1038/nmeth.2589 and PMC identifier 3771873.
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Abstract
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells.
Medical subject headings
- Gene Expression Profiling
- Optical Imaging
- Polymorphism, Single Nucleotide
- Single-Cell Analysis