Allele-specific detection of single mRNA molecules in situ.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23934076.
- Also identified by DOI 10.1038/nmeth.2601 and PMC identifier 3789122.
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Abstract
We describe a method for fluorescence in situ identification of individual mRNA molecules, allowing quantitative and accurate measurement, in single cells, of allele-specific transcripts that differ by only a few nucleotides. By using a combination of allele-specific and non-allele-specific probe libraries, we achieve >95% detection accuracy. We investigate the allele-specific stochastic expression of Nanog, which encodes a pluripotency factor, in murine embryonic stem cells.
Medical subject headings
- Alleles
- RNA, Messenger
- Single-Cell Analysis