Flow cytometric quantification of all phases of the cell cycle and apoptosis in a two-color fluorescence plot.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23935867.
- Also identified by DOI 10.1371/journal.pone.0068425 and PMC identifier 3728345.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.
Medical subject headings
- Apoptosis
- Cell Cycle
- Flow Cytometry