Downregulation of CPPED1 expression improves glucose metabolism in vitro in adipocytes.
rct · Level II
Where this comes from
- Record sourced from PubMed, PMID 23939394.
- Also identified by DOI 10.2337/db13-0830 and PMC identifier 3806586.
- Licence recorded as CC BY-NC-ND.
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Abstract
We have previously demonstrated that the expression of calcineurin-like phosphoesterase domain containing 1 (CPPED1) decreases in adipose tissue (AT) after weight reduction. However, the function of CPPED1 in AT is unknown. Therefore, we investigated whether the change in CPPED1 expression is connected to changes in adipocyte glucose metabolism. First, we confirmed that the expression of CPPED1 decreased after weight loss in subcutaneous AT. Second, the expression of CPPED1 did not change during adipocyte differentiation. Third, CPPED1 knockdown with small interfering RNA increased expression of genes involved in glucose metabolism (adiponectin, adiponectin receptor 1, and GLUT4) and improved insulin-stimulated glucose uptake. To conclude, CPPED1 is a novel molecule involved in AT biology, and CPPED1 is involved in glucose uptake in adipocytes.
Medical subject headings
- Adipocytes
- Calcineurin
- Glucose
- Weight Loss