Generating a non-integrating human induced pluripotent stem cell bank from urine-derived cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23940595.
- Also identified by DOI 10.1371/journal.pone.0070573 and PMC identifier 3734275.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived cells (UCs) under feeder-free, virus-free, serum-free condition and without oncogene c-MYC. We showed that this approach could be applied in a large population with different genetic backgrounds. UCs are easily accessible and exhibit high reprogramming efficiency, offering advantages over other cell types used for the purpose of iPS generation. Using the approach described in this study, we have generated 93 iPS cell lines from 20 donors with diverse genetic backgrounds. The non-viral iPS cell bank with these cell lines provides a valuable resource for iPS cells research, facilitating future applications of human iPS cells.
Medical subject headings
- Cell Culture Techniques
- Induced Pluripotent Stem Cells
- Urine