Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome.
basic_science · Level V
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- Record sourced from PubMed, PMID 23955771.
- Also identified by DOI 10.1038/nmeth.2603.
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Abstract
Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.
Medical subject headings
- Adenosine Diphosphate Ribose
- Aspartic Acid
- Glutamic Acid
- Poly(ADP-ribose) Polymerases
- Proteome