UPARSE: highly accurate OTU sequences from microbial amplicon reads.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23955772.
- Also identified by DOI 10.1038/nmeth.2604.
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Abstract
Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.
Medical subject headings
- Microbiota
- Phylogeny
- RNA, Ribosomal, 16S