Recombinant antibodies to histone post-translational modifications.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23955773.
- Also identified by DOI 10.1038/nmeth.2605 and PMC identifier 3828030.
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Abstract
Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.
Medical subject headings
- Antibodies
- Antibody Affinity
- Histones
- Lysine
- Protein Processing, Post-Translational