Purification of two transcription factors required for initiation by mammalian RNA polymerase II.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 2395865.
- Also identified by PMC identifier 54588.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of approximately 33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
Medical subject headings
- RNA Polymerase II
- Transcription Factors
- Transcription, Genetic