A novel method for sample delivery and testing of whole blood: gel strip PCR for point of care (POC) molecular diagnostics.

Manage, Dammika P; Lauzon, Jana; Atrazhev, Alexey; Pang, Xiaoli; Pilarski, Linda M · Lab Chip · 2013

basic_science · Level V

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Abstract

Testing of whole blood in miniaturized PCR is compromised by the opaque nature of whole blood that leads to physical masking of a fluorescent signal. We demonstrate a method to perform real-time PCR with whole blood that avoids interference from the opacity of whole blood.

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