A Tet-on system for DRD1-expressing cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23967330.
- Also identified by DOI 10.1371/journal.pone.0072681 and PMC identifier 3742517.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson's disease and addiction.
Medical subject headings
- Gene Expression Regulation
- Receptors, Dopamine D1
- Tetracycline