In vitro systems for inducers of cartilage and bone development.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 2397259.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Murine condylar cartilages of perinatal mice were cultured in the form of organ or tissue cultures on top of collagen sponges in medium containing 10% fetal calf serum. Cultures were treated with increasing concentrations of recombinant human growth hormone, recombinant IGF-I, insulin, TGF beta-1, FGF and recombinant fragments of human parathyroid hormone. All the above agents [except for the fragments rPTH (28-48) and (53-84)] were found to be mitogenic as they accelerated the proliferative activity of the condylar stem cells. IGF-I and insulin also stimulated the synthesis of cartilage proteoglycans. Growth hormone also supported de novo osteogenesis in vitro.
Medical subject headings
- Cartilage
- Growth Substances
- Osteogenesis