DMSO enhances electrospray response, boosting sensitivity of proteomic experiments.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23975139.
- Also identified by DOI 10.1038/nmeth.2610.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.
Medical subject headings
- Dimethyl Sulfoxide
- Peptides
- Proteomics
- Solvents
- Spectrometry, Mass, Electrospray Ionization
- Tandem Mass Spectrometry