A material-independent cell-environment niche based on microreciprocating motion for cell growth enhancement.

Abstract

In tissue engineering, cell-cell, cell-scaffold and cell-environment communication balances regulate how cell populations participate in tissue generation, maintenance and repair. These communication balances are called niches. In this study, an easily implemented and material-independent cell-environment niche based on microreciprocating motions is developed to enhance cell growth. A micropositioning piezoelectric lead zirconate titanate stage is used to provide precise microreciprocating shear stress motions. Various shear stresses were applied to bovine endothelial cells (BECs) that were cultured on the artificially synthesized materials to obtain the suitable shear stress for growth enhancement. It was found that the suitable shear stress for apparent enhancement of BEC growth ranges from 1.8 to 2.2 N m(-2). Biopolymers were further used to verify the feasibility of the proposed approach using the optimized shear stress obtained from the culture on artificially synthesized polymers. The results further confirmed that the growth of BECs was enhanced as expected under the calculated reciprocating frequencies based on the suitable shear stress. It is hoped that the proposed microshear-stress-based niche could be a more cost- and time-effective solution for the enhancement of cell growth in tissue engineering applications.