Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.
basic_science · Level V
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- Record sourced from PubMed, PMID 23990561.
- Also identified by DOI 10.1126/science.1240985 and PMC identifier 4359902.
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Abstract
Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.
Medical subject headings
- Cilia
- Cytoskeletal Proteins
- Muscle Proteins
- Tubulin