Absolute quantification by droplet digital PCR versus analog real-time PCR.
Where this comes from
- Record sourced from PubMed, PMID 23995387.
- Also identified by DOI 10.1038/nmeth.2633 and PMC identifier 4118677.
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Abstract
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37-86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.
Medical subject headings
- Biomarkers, Tumor
- MicroRNAs
- Real-Time Polymerase Chain Reaction