Practical innovations for high-throughput amplicon sequencing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23995388.
- Also identified by DOI 10.1038/nmeth.2634.
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Abstract
We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide. PCR clamps block amplification of contaminating sequences from a eukaryotic host, thereby substantially enriching microbial sequences without introducing bias.
Medical subject headings
- Bacteria
- Classification
- High-Throughput Nucleotide Sequencing
- Metagenome
- RNA, Ribosomal, 16S
- Soil Microbiology