DEAD box protein DDX1 regulates cytoplasmic localization of KSRP.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24023901.
- Also identified by DOI 10.1371/journal.pone.0073752 and PMC identifier 3762726.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
mRNA decay mediated by the AU-rich elements (AREs) is one of the most studied post-transcriptional mechanisms and is modulated by ARE-binding proteins (ARE-BPs). To understand the regulation of K homology splicing regulatory protein (KSRP), a decay-promoting ARE-BP, we purified KSRP protein complexes and identified an RNA helicase, DDX1. We showed that down-regulation of DDX1 expression elevated cytoplasmic levels of KSRP and facilitated ARE-mediated mRNA decay. Association of KSRP with 14-3-3 proteins, that are predominately located in the cytoplasm, increased upon reduction of DDX1. We also demonstrated that KSRP associated with DDX1 or 14-3-3, but not both. These observations indicate that subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3.
Medical subject headings
- Cytoplasm
- DEAD-box RNA Helicases
- RNA-Binding Proteins
- Trans-Activators