Three-dimensional microscaffolds exhibiting spatially resolved surface chemistry.

Richter, Benjamin; Pauloehrl, Thomas; Kaschke, Johannes; Fichtner, Dagmar; Fischer, Joachim; Greiner, Alexandra M; Wedlich, Doris; Wegener, Martin et al. · Adv Mater · 2013

basic_science · Level V

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Abstract

Spatial control over the surface chemistry of 3D organic-inorganic hybrid microscaffolds is achieved by a two-photon-triggered cycloaddition. Following a coating step with photoactivatable dienes via silanization, surface irradiation with a femtosecond-pulsed laser in the presence of functional dienophiles enables a site-selective alteration of the surface chemistry. Bioconjugation with fluorescent protein tags is employed to reveal the 3D molecular patterns.