CTR1 silencing inhibits angiogenesis by limiting copper entry into endothelial cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24039729.
- Also identified by DOI 10.1371/journal.pone.0071982 and PMC identifier 3767743.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Increased levels of intracellular copper stimulate angiogenesis in human umbilical vein endothelial cells (HUVECs). Copper transporter 1 (CTR1) is a copper importer present in the cell membrane and plays a major role in copper transport. In this study, three siRNAs targeting CTR1 mRNA were designed and screened for gene silencing. HUVECs when exposed to 100 µM copper showed 3 fold increased proliferation, migration by 1.8-fold and tube formation by 1.8-fold. One of the designed CTR1 siRNA (si 1) at 10 nM concentration decreased proliferation by 2.5-fold, migration by 4-fold and tube formation by 2.8-fold. Rabbit corneal packet assay also showed considerable decrease in matrigel induced blood vessel formation by si 1 when compared to untreated control. The designed si 1 when topically applied inhibited angiogenesis. This can be further developed for therapeutic application.
Medical subject headings
- Cation Transport Proteins
- Copper
- Human Umbilical Vein Endothelial Cells
- Neovascularization, Physiologic