A two-component system regulates hemin acquisition in Porphyromonas gingivalis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24039921.
- Also identified by DOI 10.1371/journal.pone.0073351 and PMC identifier 3764172.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Porphyromonas gingivalis is a Gram-negative oral anaerobe associated with infection of the periodontia. The organism has a small number of two-component signal transduction systems, and after comparing genome sequences of strains W83 and ATCC 33277 we discovered that the latter was mutant in histidine kinase (PGN_0752), while the cognate response regulator (PGN_0753) remained intact. Microarray-based transcriptional profiling and ChIP-seq assays were carried out with an ATCC 33277 transconjugant containing the functional histidine kinase from strain W83 (PG0719). The data showed that the regulon of this signal transduction system contained genes that were involved in hemin acquisition, including gingipains, at least three transport systems, as well as being self-regulated. Direct regulation by the response regulator was confirmed by electrophoretic mobility shift assays. In addition, the system appears to be activated by hemin and the regulator acts as both an activator and repressor.
Medical subject headings
- Bacterial Proteins
- Bacteroidaceae Infections
- Hemin
- Host-Pathogen Interactions
- Porphyromonas gingivalis
- Protein Kinases