Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24056873.
- Also identified by DOI 10.1038/nmeth.2647 and PMC identifier 3860264.
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Abstract
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.
Medical subject headings
- Calcium Signaling
- Cilia