Inducible control of gene expression with destabilized Cre.
basic_science · Level V
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- Record sourced from PubMed, PMID 24056874.
- Also identified by DOI 10.1038/nmeth.2640 and PMC identifier 3947879.
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Abstract
Acute manipulation of gene and protein function in the brain is essential for understanding the mechanisms of nervous system development, plasticity and information processing. Here we describe a technique based on a destabilized Cre recombinase (DD-Cre) whose activity is controlled by the antibiotic trimethoprim (TMP). We show that DD-Cre triggers rapid TMP-dependent recombination of loxP-flanked ('floxed') alleles in mouse neurons in vivo and validate the use of this system for neurobehavioral research.
Medical subject headings
- Gene Expression Regulation
- Integrases