Design of a single-step immunoassay principle based on the combination of an enzyme-labeled antibody release coating and a hydrogel copolymerized with a fluorescent enzyme substrate in a microfluidic capillary device.
basic_science · Level V
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- Record sourced from PubMed, PMID 24064761.
- Also identified by DOI 10.1039/c3lc50775k.
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Abstract
A combination of an enzyme-labeled antibody release coating and a novel fluorescent enzyme substrate-copolymerized hydrogel in a microchannel for a single-step, no-wash microfluidic immunoassay is demonstrated. This hydrogel discriminates the free enzyme-conjugated antibody from an antigen-enzyme-conjugated antibody immunocomplex based on the difference in molecular size. A selective and sensitive immunoassay, with 10-1000 ng mL(-1) linear range, is reported.
Medical subject headings
- Antibodies
- Enzymes
- Fluorescent Dyes
- Hydrogel, Polyethylene Glycol Dimethacrylate
- Immunoassay
- Microfluidic Analytical Techniques