Contrasting quiescent G0 phase with mitotic cell cycling in the mouse immune system.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24066072.
- Also identified by DOI 10.1371/journal.pone.0073801 and PMC identifier 3774768.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
A transgenic mouse line expressing Fucci (fluorescent ubiquitination-based cell-cycle indicator) probes allows us to monitor the cell cycle in the hematopoietic system. Two populations with high and low intensities of Fucci signals for Cdt1(30/120) accumulation were identified by FACS analysis, and these correspond to quiescent G0 and cycling G1 cells, respectively. We observed the transition of immune cells between quiescent and proliferative phases in lymphoid organs during differentiation and immune responses.
Medical subject headings
- Hematopoietic System
- Resting Phase, Cell Cycle