Bond elasticity controls molecular recognition specificity in antibody-antigen binding.

Alemany, Anna; Sanvicens, Nuria; de Lorenzo, Sara; Marco, M-Pilar; Ritort, Felix · Nano Lett · 2013

basic_science · Level V

Where this comes from

Abstract

Force-spectroscopy experiments make it possible to characterize single ligand-receptor pairs. Here we measure the spectrum of bond strengths and flexibilities in antibody-antigen interactions using optical tweezers. We characterize the mechanical evolution of polyclonal antibodies generated under infection and the ability of a monoclonal antibody to cross-react against different antigens. Our results suggest that bond flexibility plays a major role in remodeling antibody-antigen bonds in order to improve recognition during the maturation of the humoral immune system.

Medical subject headings