Rare-earth-incorporated polymeric vector for enhanced gene delivery.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24103650.
- Also identified by DOI 10.1016/j.biomaterials.2013.09.069.
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Abstract
Cationic polymer PEI-CyD is doped with Nd by plasma technology to produce the gene vector: Nd@PEI-CyD. Luciferase expression and EGFP transfection experiments performed in vitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD and the mechanism is studied and proposed. The rare-earth element, Nd, stimulates the energy metabolism of cells, enhances cell uptake of complexes/pDNA, and regulates the cellular pathways. These special features suggest a new strategy involving metal-incorporated non-viral gene vectors.
Medical subject headings
- Gene Transfer Techniques
- Genetic Vectors
- Metals, Rare Earth
- Polymers