Nano-anchors with single protein capacity produced with STED lithography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24111646.
- Also identified by DOI 10.1021/nl4033523.
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Abstract
Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.
Medical subject headings
- Acrylates
- Antibodies
- Nanostructures
- Nanotechnology