Detergent-free mass spectrometry of membrane protein complexes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24122040.
- Also identified by DOI 10.1038/nmeth.2691 and PMC identifier 3868940.
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Abstract
We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.
Medical subject headings
- Detergents
- Lipids
- Mass Spectrometry
- Membrane Proteins
- Micelles