Time-lapse AFM imaging of DNA conformational changes induced by daunorubicin.

Alonso-Sarduy, Livan; Longo, Giovanni; Dietler, Giovanni; Kasas, Sandor · Nano Lett · 2013

basic_science · Level V

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Abstract

Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. In this work, we show how atomic force microscopy in liquid can characterize, through time-lapse imaging, the dynamical influence of intercalating agents on the supercoiling of DNA, improving our understanding of the drug's effect.

Medical subject headings