Genomically recoded organisms expand biological functions.
basic_science · Level V
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- Record sourced from PubMed, PMID 24136966.
- Also identified by DOI 10.1126/science.1241459 and PMC identifier 4924538.
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Abstract
We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.
Medical subject headings
- Amino Acids
- Bacteriophage T7
- Codon, Terminator
- Escherichia coli
- Organisms, Genetically Modified