Metagenomic species profiling using universal phylogenetic marker genes.
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- Record sourced from PubMed, PMID 24141494.
- Also identified by DOI 10.1038/nmeth.2693.
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Abstract
To quantify known and unknown microorganisms at species-level resolution using shotgun sequencing data, we developed a method that establishes metagenomic operational taxonomic units (mOTUs) based on single-copy phylogenetic marker genes. Applied to 252 human fecal samples, the method revealed that on average 43% of the species abundance and 58% of the richness cannot be captured by current reference genome-based methods. An implementation of the method is available at http://www.bork.embl.de/software/mOTU/.
Medical subject headings
- Metagenomics
- Microbiota
- Sequence Alignment