Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24146020.
- Also identified by DOI 10.1039/c3lc50736j.
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Abstract
The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, we demonstrate a general method to harness flow cytometry, with its unmatched speed and sensitivity, for droplet-based microfluidic sorting.
Medical subject headings
- Flow Cytometry
- Microfluidic Analytical Techniques