Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
basic_science · Level V
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- Record sourced from PubMed, PMID 24146923.
- Also identified by DOI 10.1371/journal.pone.0076751 and PMC identifier PMC2715653.
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Abstract
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.