Semiconductor-based DNA sequencing of histone modification states.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24157732.
- Also identified by DOI 10.1038/ncomms3672 and PMC identifier 3917140.
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Abstract
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
Medical subject headings
- Genome, Human
- Histones
- Melanoma
- Protein Processing, Post-Translational
- Sequence Analysis, DNA
- Skin Neoplasms