The need for transparency and good practices in the qPCR literature.

Bustin, Stephen A; Benes, Vladimir; Garson, Jeremy; Hellemans, Jan; Huggett, Jim; Kubista, Mikael; Mueller, Reinhold; Nolan, Tania et al. · Nat Methods · 2013

review · Level V

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Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

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