Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24185478.
- Also identified by DOI 10.1039/c3lc50945a.
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Abstract
Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.
Medical subject headings
- Cytokines
- Microfluidic Analytical Techniques
- T-Lymphocytes