A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
basic_science · Level V
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- Record sourced from PubMed, PMID 24204723.
- Also identified by DOI 10.1371/journal.pone.0077021 and PMC identifier PMC3273792.
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Abstract
We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.