Quantifying the local resolution of cryo-EM density maps.
basic_science · Level V
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- Record sourced from PubMed, PMID 24213166.
- Also identified by DOI 10.1038/nmeth.2727 and PMC identifier 3903095.
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Abstract
We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.
Medical subject headings
- Cryoelectron Microscopy
- Proteins