ClpX shifts into high gear to unfold stable proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24243009.
- Also identified by DOI 10.1016/j.cell.2013.10.007.
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Abstract
Protein degradation by the ClpXP protease requires collaboration among the six AAA+ domains of ClpX. Using single-molecule optical tweezers, Sen et al. show that ClpX uses a coordinated succession of power strokes to translocate polypeptides in ATP-tunable bursts before reloading with nucleotide. This strategy allows ClpX to kinetically capture transiently unfolded intermediates.
Medical subject headings
- Endopeptidase Clp
- Escherichia coli
- Escherichia coli Proteins