Simultaneous expression of displayed and secreted antibodies for antibody screen.
basic_science · Level V
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- Record sourced from PubMed, PMID 24244593.
- Also identified by DOI 10.1371/journal.pone.0080005 and PMC identifier PMC1964754.
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Abstract
The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through fluorescence-activated cell sorting (FACS) analysis, as well as in conditioned medium in a secreted version for function analysis.