A quantitative liposome microarray to systematically characterize protein-lipid interactions.

Saliba, Antoine-Emmanuel; Vonkova, Ivana; Ceschia, Stefano; Findlay, Greg M; Maeda, Kenji; Tischer, Christian; Deghou, Samy; van Noort, Vera et al. · Nat Methods · 2014

basic_science · Level V

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Abstract

Lipids have a role in virtually all biological processes, acting as structural elements, scaffolds and signaling molecules, but they are still largely under-represented in known biological networks. Here we describe a liposome microarray-based assay (LiMA), a method that measures protein recruitment to membranes in a quantitative, automated, multiplexed and high-throughput manner.

Medical subject headings