Quantifying RNA allelic ratios by microfluidic multiplex PCR and sequencing.
basic_science · Level V
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- Record sourced from PubMed, PMID 24270603.
- Also identified by DOI 10.1038/nmeth.2736 and PMC identifier 3877737.
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Abstract
We developed a targeted RNA sequencing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniformly and simultaneously amplify up to 960 loci in 48 samples independently of their gene expression levels and to accurately and cost-effectively measure allelic ratios even for low-quantity or low-quality RNA samples. We applied mmPCR-seq to RNA editing and allele-specific expression studies. mmPCR-seq complements RNA-seq for studying allelic variations in the transcriptome.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Multiplex Polymerase Chain Reaction
- RNA
- Sequence Analysis, RNA