MicroRNA-33 regulates sterol regulatory element-binding protein 1 expression in mice.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 24300912.
- Also identified by DOI 10.1038/ncomms3883 and PMC identifier 3863899.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
MicroRNAs (miRs) are small non-protein-coding RNAs that bind to specific mRNAs and inhibit translation or promote mRNA degradation. Recent reports have indicated that miR-33, which is located within the intron of sterol regulatory element-binding protein (SREBP) 2, controls cholesterol homoeostasis and may be a potential therapeutic target for the treatment of atherosclerosis. Here we show that deletion of miR-33 results in marked worsening of high-fat diet-induced obesity and liver steatosis. Using miR-33(-/-)Srebf1(+/-) mice, we demonstrate that SREBP-1 is a target of miR-33 and that the mechanisms leading to obesity and liver steatosis in miR-33(-/-) mice involve enhanced expression of SREBP-1. These results elucidate a novel interaction between SREBP-1 and SREBP-2 mediated by miR-33 in vivo.
Medical subject headings
- Fatty Liver
- Gene Expression Regulation
- MicroRNAs
- Obesity
- Sterol Regulatory Element Binding Protein 1